PCR TEST (MOLECULAR TEST)

The highly conserved gene fragment of ORF1ab and N of 2019-nCoV are selected as the target to design special primers and Taqman probes. The conserved gene fragment of ORF1ab and N will be amplified accurately by specific primers, and detected by the taqman probes. During the process of PCR amplification, the Taq enzyme will cut off the fluorophore (at the 5′ end) from the pro

The highly conserved gene fragment of ORF1ab and N of 2019-nCoV are selected as the target to design special primers and Taqman probes. The conserved gene fragment of ORF1ab and N will be amplified accurately by specific primers, and detected by the taqman probes. During the process of PCR amplification, the Taq enzyme will cut off the fluorophore (at the 5′ end) from the probe, so that the fluorophore can dissociate in the reaction system and get rid of the shielding of quencher (at the 3′ end). Then, the fluorophore will be stimulated by light and emit fluorescence which can be detected by instruments. In this way, 2019-nCoV nucleic acid can be detected in the fully automatic enclosed reaction system. The internal control in the kit was used a specific primer for a pair of housekeeping genes and specific probes. The probe can specifically combine with a segment of nucleic acid template in the region of primer amplification. The internal control selects HEX/VIC channel. Thus, the monitoring process in the closed reaction system can be realized and the occurrence of false negatives can be monitored effectively